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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guideline outlines even further doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which lead into the temporal coordination of each mitotic function. Cyclin E varieties a posh with and functions like a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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